User:Shellandsea

Summary of Qualifications

•	Excellent data gathering, research and follow through with communication, documentation, technical skills created on time actions saved time and money to reduce the downtown of the machine(s). •	Achieved the bioassay development with a strong chemistry/biochemistry knowledge to find matching solution, enzymes and buffers, honed my skills into preparing adequate samples to be analyzed by knowing the working limits, specifics, and technology of the machines, cell sorter, FACS, microscopy, PCR. Comprehensive experience to do Interdisciplinary studies established by weighing the pros-cons for connecting the sample-out of specifics (OOS)-validation-analysis-products/machines on biological outcome.

•	 Industrial microbiology in the lab and at the field with internships with pharmaceutical, food processing, and health monitoring fields to develop and apply  scale up and QC  applications.

•	Affected by energetic, versatile, enthusiastic, influential conversed science among diverse backgrounds to teach, train, and monitor.

•	Scheduled and coordinated individuals according to their specific needs to develop, find solution to their shortcomings.

•	Developed liaisons among industry, academia and business. Easy to build rapport. Effective problem solver.

•	Planned, organized, and delegated responsibility for several projects.

•	Established objectives for a yearly budget, which involved balancing the daily routine work, preliminary data for unfunded projects.

•	Talented and initiator with energetic, dependable, loyal and likable personality. Created sense of direction with a clear communication.

•	Recognized to organize, create collaborations, and plan.

•	DNA, RNA, protein analysis and expression, microbial testing, screen tests, SDS-PAGE, FACS, ELISA, EMSA, Flow cytometry, IHC, FITC, Lowry, Bradford, staining, biochemistry tests, enzyme analysis, PCR, cell culture, animal models, surgery, anesthesia, microarray, OOS, GMP, SOP, bioassay development, microscopy, confocal, light, fluorescent, bioinformatics, statistics.

Accomplishments and Honors: •	North Atlantic Treaty Organization (NATO) Advanced Science Institute (ASI) Grant for "Genome Structure and Function" in Elba Island, ITALY (1996). •	National Merit Scholarship, Government of Turkey, 1990-1998 •	British Council Graduate Study Scholarship (1990). •	Japanese Government Ministry of Education Scholarship (1990), •	Science Faculty, Ege University, Summa cum Laude (1990). •	Valedictorian in Department of Biology 1990

BIOSENSORS AND BIOMARKERS •	The project goal was to preserve B cells that are used in CANARY technique as biosensors to detect pathogens. Collaborated with ECU, MIT and a private company. •	Preserved B sensitized cells to be used immediate reaction for immune defense, validate experiments, trained a technician. Applied fixation, cell signaling, confocal microscopy, cell viability, and apoptosis. •	Lyophilization, transfer technology, SOP, GMP, OOC, bioassay development. Reported to VP, CEO. Wrote reports and present data on validation and instrumental activities.

GENE THERAPY AND HEMOSTASIS •	Accomplished viral and non-viral gene therapy to treat Hemophilia B.  Delivered the transgenes FIX and VKOR by intravascular or intramuscular, and novel sine wave electrophoresis techniques to neonate and adults. Showed VKOR improved the FIX specific activity. •	 Applied GMP, SOP, protein analysis, ELISA, FACS, Genetics, molecular therapy, coagulation, Vitamin K cycle, Human Factor IX, VKOR, coagulation tests, ELISA, sequential immunofluoresce staining, confocal microscopy, and molecular cloning, double gene constructs, immune response.

THROMBOSIS AND CARDIOVASCULAR BIOLOGY •	Defined the cellular differentiation mechanisms due to hemostatic agents. Proved bovine thrombin increased the proliferation of transplanted grafts, resulted early replacement of the grafts. Showed that aprotinin has specificity on endothelial cell coagulation biology during cardiopulmonary bypass (CPB) on PAR. •	Protein analysis, transplant rejections, SOP, OOS, SDS-PAGE, enzyme function, cell culture, animal surgery, support instrument and validation activities Trained personal residences, research fellow, technician, and students.

ONCOLOGY Liquid Cancer Models: Leukemia and myeloma •	Analyzed genetic regulation during T cell Activation, IL-2 proliferation. Used transcription regulation and mutagenesis for ATF-4, p300, CREB. T cell lines. Microarray Analysis with Affymetrix chips

Solid Cancer Models: Prostate and Breast Cancer •	Studied the prevention of metastasis and understand the microenvironment. Delaminated stroma secreted a soluble protein influenced endothelial cell differentiation to develop tumors.

GENE TARGETING for a PHARMACEUTICAL ANTI-FUNGAL DRUG •	Established a target gene in asexual reproduction of Aspergillus nidulans to prevent fungal disease. Demonstrated epitope tagging, site directed mutagenesis, PCR, and DNA cloning technology. Wrote SOP, validated instruments, technical writing, stability testing.

STEM CELLS AND GENE REGULATION •	Found the transactivation of ovo female germ line specific Drosophila melanogaster gene by deletion mutations of X-chromosome. Completed complex genetic screening and identification/characterization of transcriptional regulatory mechanisms governing Drosophilia germline and system biology. Discovered first time in the scientific community that there are two auto-regulation mechanisms for ovo to control germline sex determination. •	Presented in the International Drosophila Meeting 1997 (Chicago) •	Developed bioassays. Applied molecular and classical genetic tools, stem cell, mutagenesis, deletion mapping, transcriptional control, gene interactions, in vivo and in vitro, transgenetics. Analyzed protein expression and enzyme activities, spectrophotometry, and histology. Acquired statistics, SAS, MATLAB, and Minitab. Supported instrument validation and support.

MICROBIOLOGY •	Minimized the use of Vitreoscilla hemoglobin gene promoter to insert more metabolism genes to synthesize more industrial products in E.coli. •	Compared the growth of Echerichia coli constructs under the low oxygen to increase the fermentation product quality, reduced the change of tank in the manufacturing facility. •	Applied microbiologic testing methods for public health in Environmental Health, Food Microbiology, Mycology, Enterobacteriology, and Serology. •	Honed molecular biology and microbial engineering for the biotech, DNA technology, protein analysis methods in human specimens, ELISA, RIA, serological and immunological tests, microbial physiology screening tests and techniques.

TEACHING EXPERIENCE Thought several years’ at all educational levels from high school to university in diverse settings, like inner-city Chicago, a rural city Alamance in North Carolina, a competitive Istanbul. Experienced in variety of learning styles. Committed to provide a comfortable environment to grow and explore ideas were the common thread in all of these experiences to develop critical and problem solving skills in science. .

Present            Bahcesehir University Faculty of Engineering Besiktas Istanbul Faculty, Assistant Professor -	Teaching MDE Human Anatomy and Physiology for Medical Engineering students -	Establishment of Medical Engineering Department within Faculty of Engineering. -    I am managing several -research projects: -	Cardiovascular research with Hacettepe University Medical Center, Ankara. -	Gene therapy and stem cell application for diabetes with Ege University Medical Center, Izmir. -	Neural cell differentiation and hemostasis with Yeditepe University Medical Center, Istanbul. -	Cancer prevention drugs for cancer with Yeditepe University Medical Center, Istanbul.

Present               University of Phoenix, Raleigh Campus Associated Faculty -   HPE170 Health and Physical Education -	SCI 100 Paradigms of Health -	SCI 201 Survey of Alternative Medicine -	SCI 220 Human Nutrition -	BIOL 100 Biology Summer 2008                  Alamance Community College, Graham, NC                                         Instructor - Thought “Biol 112 General Biology II”.

Faculty -Molecular Biology and Genetics •	Taught graduate courses in Genes and Development, Biochemistry, General Biology. •	Mentored and advised graduate students on their research.

Tutor •	Tutored honors and advanced program in Science, Mathematics, Geometry, Algebra, Physics, Biology, and Chemistry for ACT test at honors and AP levels and followed up with their parents. •	Monitored and organized extracurricular activities. Succeeded in improving students learning and successes in their course work and exams.

Teaching Assistant •	Taught classical genetics techniques, and recombinant DNA technology, isolating, cloning, screening, labeling, blotting, PCR, preparing gels. 35 students (303 Genetics Lab) •	Taught experiments of fundamental chemistry laws, techniques to measure and analyze data for 50 students in two classes. (101 General Chemistry).

WORK EXPERIENCE Aug 2008-Present Bahcesehir University, Faculty of Engineering, Besiktas-Istanbul Turkey Assistant Prof. Apr. 2007- Present University Phoenix, Raleigh, NC Associated Faculty Dec 2006-Nov 2007 Department of Pathology, Faculty/Research Associate, Eastern Carolina University  Greenville, NC Mar 2005-Dec 2006 Gene Therapy Center Postdoctoral Research Associate, University of North Carolina, Chapel Hill, NC March 2002–Oct 2004 Research Associate, Duke University Medical Center Department of Surgery, Durham, NC      June 2000-March 2002 Faculty -Molecular Biology Izmir Institute of Technology, Gulbahce Koyu, Turkey Sept 1999 - May 2000 Visiting Postdoctoral Fellow, National Institute of Health/National Cancer Institute, Advanced Technology Center, Department of Pathology, Bethesda, MD Dec 1998 - Aug 1999 Postdoctoral Researcher, Southern Illinois University (SIU) School of Medicine, Springfield, IL   Dec 1993 - Dec 1998 Graduate Research Assistant Illinois Institute of Technology, Chicago, IL   Aug 1992 – Dec 1993 Graduate Research Assistant Illinois Institute of Technology, Chicago, IL   Oct 1993 - May 1995 Tutor Columbia College, Upward Bound Program, Chicago, Illinois Jan 1994 - Dec 1998 Teaching Assistant, Illinois Institute of Technology, Chicago, IL                1989 summer Research Intern, Refik Saydam Public Health Institute, Izmir, Turkey

EDUCATION AND TRAINING Ph.D., Biology  Illinois Institute of Technology, Chicago, IL, Dec. 1998 MS, Biology      Illinois Institute of Technology, Chicago, IL, Dec. 93 B.S., Biology     Ege University, Science Faculty, Bornova/ Turkey, Jun. 90 Sum cum Laude •	Postdoctoral Research Associate, University of North Carolina at Chapel Hill, Gene Therapy Center (Mar. 05-Dec 2006) •	Research Associate, Duke University Medical Center, Durham, NC (Mar 02 – Oct 04) •	Visiting Postdoctoral Fellow, NIH/NCI, Bethesda, MD, (Sept. 99 - May 00). •	Postdoctoral Fellow, SIU School of Medicine, Springfield, IL (Dec. 98 - Aug. 99)

PRESENTATIONS -	Yeditepe University Genetic Eng. and Bioengineering, Kozyatağı Istanbul 2008 -	Sabancı University Engineering and Natural Sciences Molecular Genetic Department,Istanbul 2008 -	TUBITAK Genetic Engineering Institute Turkish Science and Research Institute, Gebze 2008 -	Istanbul Kültür University, Molecular Genetics Dept, 2008 -	University of Phoenix, Raleigh NC (2008) -	BRITE North Carolina Central University, Durham, NC (2008) -	Wake Tech Community College, Raleigh, NC (2007) -	University of North Carolina, Chapel Hill (2006) -	Nicholas State University (2004) -	Duke University Medical Center, Durham NC (2002) -	Bosphorus University, Istanbul Turkiye (2000) -	Izmir Institute of Technology, Izmir, Turkiye (2000) -	41st Annual International Drosophila Research Conference, (2000) -	Illinois Institute of Technology, Chicago, IL. (1998) -	38th Annual International Drosophila Research Conference Chicago, IL (1997) -	University of Pennsylvania at Philadelphia, PA, (1996)

COMPUTER SKILLS •	Database and programming languages: Access, C++. •	Engineering math/statistical packages: SAS, MiniTab, Visual. •	Graphics and other programs: Microsoft Project, Micrografx Designer, Microsoft Power Point, Microsoft Visio, Stanford Graphics, Sigma Plot, Freelance, Adobe Photoshop.

PROFESSIONAL AFFILIATIONS •	American Association for the Advancement of Science (AAAS) •	Genetics Society of America (GSA) •	Society of Developmental Biology (SDB) •	The American Society of Gene Therapy (ASGT) •	The American Society of Microbiology (ASM)